Antitumor effect of para-toluenesulfonamide against lung cancerxenograft in a mouse model

Background: Conventional chemotherapy and radiation therapy against non-small cell lung cancer (NSCLC) are relativelyinsensitive and unsatisfactory. ara-toluenesulfonamide (PTS), a unique antitumor drug for local intratumoral injectionshows an eficacy of severely suppressing solid tumor growth with mild side effects in clinical trials. The aim of this studwas to investigate the effect ofPTS on lung cancer H460 cells in vivo in nude mice and its underlying mechanisms in vitro.Methods: A lung cancer model for in vivo experiment was established in BALB/c nude mice using H460 cells to examinehe effect oflocal injection ofPTS on tumor suppression, We also assessed the injury to the normal tissue by subcutaneousinjection of PTS. In vitro, PTS was diluted into different doses for study on its antitumor mechanisms.We evaluated thenecrotic effect of PTS on H460 cells by Pl and Hoechst 33342 staining. Cell viability and membrane permeability were alsoletermined by using CCK-8 and LDH assays respectively, All these tests were conducted in comparison with traditionaocal iniection of anhydrous ethanol.

Results: PTS was shown to signifcantly inhibit the growth of H460 tumor xenografts in nude mice by inducing necrosiof the tumor histologically lts effect on tumor growth was significantly stronger than that of anhydrous ethanol. Brcontrast, the injured normal tissue by PTS injection was less than that by ethanol. In vitro, PTS still demonstrated excellentnecrotizing efect on H460 cells when diluted to a lower concentration, Detailed analysis of PTS on H460 cells indicatedhat PTS had a better effect on attenuating the cell viability and increasing the cel membrane permeability than ethanol athe same level

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